4000 bp - 75ng
2000 bp - 200ng
1000 bp - 100ng
500 bp - 50 ng
100 bp - 20ng
1 ml of premixed DNA marker with loading dye.
A. Mix thoroughly by inverting tube 2-3 times before use.
B. Load 10ėl per lane. Run in 1.5% agarose gel at 200 constant volts.
Based on 1ėg of marker per application in a volume of 10ėl (including
dye). DNA concentration per band is as indicated. Gel was run through 14cm long
gel, in TAE buer containing Ethidium Bromide (EtBr), and visualized at the
365nm. Loading dye consists of bromophenol blue. For research use only. Not
intended for animal or human diagnostic or therapeutic use.
The DNA band quantities listed above are determined by spectrophotometer, but
lot to lot variation is clearly apparent.
Residual ammonium ions are present and may inhibit kinase exchange reactions.
Both marker and dye contain 0.02% sodium azide.
DNA Amount (ng) per Band
100 Applications (with loading dye)
Also available without loading dye.