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Custom Oligo's by email
 
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BIONEXUS- Services

 

OLIGO SYNTHESIS SERVICES

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Synthesis
Modifications
Prices

siRNA Synthesis Services

DESCRIPTION OF SERVICES

Oligonucleotide Synthesis and Purification Services
Bionexus provides the highest quality and the widest variety of custom oligonucleotide synthesis of modified, labeled, fluorogeneic 5' nuclease probes and phosphorothioate oligonucleotides. All with our famous NoMutantsTM guarantee. This guarantee ensures that your oligonucleotide will be delivered as specified or it will be re-made for you free of charge.

All Oligo Syntheses Include:
DNA Synthesis
Bionexus oligonucleotides are synthesized using the latest DNA chemistries to guard against depurination and breakdown of modifications during ammonia cleavage and deprotection. Oligonucleotides are available in the 50 nanomole, 0.2 micromole, 1.0 micromole, 10 micromole and larger scales.

Quantity Delivered
The quantity of modified oligonuleotide you receive from Bionexus is a function of the synthesis scale, type of modification, position of modification and purification method. Below is a table with the of the range of product yields that can be expected:

Crude DNA Synthesis Yield
Synthesis Scale (micromoles)
0.05
0.2
1.0
10.0
Crude
5-15 ODs
12-30 ODs
60-100 ODs
400-600 ODs
HPLC
---
3-10 ODs
20-35 ODs
130-200 ODs
PAGE
---
1-3 ODs
6-10 ODs
40-50 ODs


Purification
Bionexus offers both HPLC and PAGE purification to remove the n - 1 and n + 1 sequences that can be generated during synthesis. The selection of purification method depends on the type of oligonucleotide and on your purity and yield requirements. There is a trade-off between purity and yield. The higher the purity, the smaller the yield and the lower the purity, the higher the yield.
HPLC purification generally gives higher yields but lower purity than PAGE purification. HPLC will deliver > 90 % purity and PAGE > 95 % purity as measured by area percentage of product peak or band respectively. We also offer unique HPLC purification schemes for AP and HRP and for dual label fluorogenic 5' nuclease probe assay.



Oligonucleotide Modifications

Bionexus offers the widest variety of high quality modifications in the DNA synthesis industry. We offer just about every fluorescent dye in existence for DNA oligonucleotide labeling. These include the Molecular Probes dyes, and FAM, TAMRA, HEX, TET and JOE in a dual label configuration. We have the enzyme conjugates Alkaline Phosphatase (AP) and Horse Radish Peroxidase (HRP). We also make phosphorothioate and mixed base oligonucleotides on a regular basis.

Fluorescent Dye Labels
On the following page are a list of some of the dyes we offer for oligonucleotide labeling. These can be placed either on the 5' end, the 3' end, or internally. If there is a label or modification you would like to use but you do not see it here, please contact us, we may have it or we may be able to locate it for you.

Enzyme Conjugates
Bionexus has provided high quality and highly purified Alkaline Phosphatase (AP) and Horse Radish Peroxidase (HRP) enzyme conjugates to researchers and product manufacturers for many years. We perform a number of tests to ensure that the enzyme conjugates are of high quality including specific activity and blot tests. We can also send you the AP or HRP conjugate probes in a concentration and storage buffer that you specify. Please contact us for details.

Dual Label Fluorogenic 5' Nuclease Assay Probes
Bionexushas extensive experience in the synthesis and optimization of dual labeled 5' nuclease probes. We have perfected synthesis, purification and quality control tests for these types of oligonucleotides which are currently being used by researchers worldwide.

In addition to the standard method of attaching the reporter dye to the 5' end and the quencher dye on the 3' end, Bionexuscan offer the placement of any of the dye options listed below at either the 5', 3' or internal positions. Please contact us if you have questions about ideas for the optimization of your probe.

Reporter Dyes - 5'
6-FAM (496/516)
HEX (535/556)
TET (521/536)
Quencher Dyes - 3'
TAMRA (565/580)
JOE (520/548)

Bionexus provides these ABI PrizmTM probes in two scales - the 0.2 micromole test scale (1-3 nanomole yield for the trial and error phase of your project) for $350 and the larger 1.0 micromole scale (yield of > 20 nanomoles) for your larger experiments for $650.


Ordering a Custom Oligo has never been so easy!

Click here to email us your order for the quickest turnaround



OLIGO SYNTHESIS SERVICES - PRICES
To Order or Request a Quote just Click below

SYNTHESIS
NO SET-UP CHARGE!
Your order will ship to you Deprotected and Desalted and includes a Synthesis Report & Gel Picture.

Product Synthesis
Scale
Price*
Normal Diester DNA 0.05 Ámol
.2 Ámol
1 Ámol
10 Ámol
100-500 Ámol
$0.45/base
$0.80/base
$2.00/base
$14.00/base
Inquire
Phosphorothioate DNA

0.2 umol

1 Ámol
15Ámol
100-500 Ámol

$2.00/base

$4.00/base
$20.00/base
Inquire

RNA 1 Ámol $20.00/base
RNA Thioate 1 Ámol $25.00/base
2' O-Methyl RNA 200 Ámol
10 Ámol
$30.00/base
Inquire
*Price includes setup, synthesis, deprotection, and QC data. Discounts available for blanket purchase agreements, standing orders, and large special orders.


PURIFICATION - help? -

Micromole

0.05


0.2


1.0

10.0

 

DeSalt FREE FREE FREE FREE
Cartridge $16 $16 $16 Inquire
HPLC $50 $50 $75 $500
PAGE $75 $75 $100 $900
EconoPure FREE FREE FREE FREE

Cartridge and HPLC purification are most effective for oligos less than 40 bases. PAGE is recommended for oligos longer than 40 bases.


MODIFICATIONS AND CONJUGATES

Synthesis Scale and Price**
Modifications 3' 5' 0.2
Ámol
1.0 Ámol 10.0 Ámol
FITC
FAM
HEX
TET
TAMRA
JOE
ROX
Texas Red
Cy5
Cy3
Amine
Phosphate
Biotin
Rhodamine
TMR
Bodipy
Cascade Blue
Oregon Green
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$50
$100
$100
$100
$100
$100
$100
$100
$100
$100
$60
$60
$60
$50
$200
$200
$200
$200
$75
$150
$150
$150
$150
$150
$150
$150
$150
$150
$100
$100
$90
$90
$300
$300
$300
$300
$300
$400
$400
$400
$400
$400
Inquire
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$300
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Conjugates
Horseradish Peroxidase (HRP)
Alkaline Phosphatase (AP)
Price***
$425
$585
** Price includes derivatization or conjugation and QC data.
*** Add cost of custom oligo synthesis using 1 Ámol scale

Dual Label 5' Nuclease Probes 2-5 nanomoles - $ 350
15+ nanomoles - $ 650




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