Bionexus uses Laser micromanipulation from paraffin-embedded or
frozen tissues using a PALM MicroBeam HT platform (Zeiss MicroImaging Group,
Thornwood, NY) allows selecting tissue regions or cell types of interest and
eliminates the analysis of unwanted bystander tissue. A cold UV-laser with a
focus point of 1 µm removes the selected object by a process called cold
ablative photolysis. Cells are removed from the context without generating
heat by disintegrating the chemical bonds between atoms. The PALM MicroBeam
HT allows non-contact sample handling and minimizes cross-contamination during
tissue removal. Using the same laser, the selected sample is then catapulted
and transported directly into a collection device.
A selection of specific applications includes
Classical tissue microdissection
Mark you target tissue and isolate it without contamination of unwanted bystander
The PALM MicroLaser System retains the viability of catapulted cultured cells
or other organisms and can be used for biological sub-cloning within a cell
culture dish (stem cell purification).
Single chromosomes or chromosomal parts can be isolated from metaphase preparations
for subsequent molecular analysis.
Sperm can be isolated from a mucus smear obtained from rape victims for subsequent
Selection and capturing of hard phloem can be conducted with preserved morphology
after PALM MicroLaser manipulation.
Use catapulted sections for RNA extraction and subjected to direct Microarray
Catapulted samples can be used for protein analysis, e.g. on a SELDI protein
All communications for this Service will be held in the strictest confidence.
Contact Bionexus for a price
quotation and more information today.