SYBER® Green Chemistry
Quantitative PCR is a powerful, highly sensitive technique that
can be used to quantitate gene expression, determine gene copy number, detect
SNPs, and detect DNA from viral and bacterial microorganisms.
Bionexus utilizes Applied Biosystems 7900HT and 5700 Sequence Detection Systems
to perform qPCR experiments. Our 7900HT system is equipped with both 96-well
and 384-well blocks to facilitate high throughput analysis. We support both
Syber Green and Taqman reaction chemistries. With Taqman chemistry, a labeled
probe is included in the reaction to enhance the specificity and sensitivity
of target sequence detection. With SyberGreen chemistry, the amplified product
is detected by its interaction with the SyberGreen fluorescent dye, which selectively
binds to double-stranded DNA templates. SyberGreen chemistry is cost efficient
but generally requires more effort to optimize reactions.
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