Taqman Services - Gene Expression (Quantitative PCR) Inquire for pricing
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Code: Taqman Services(Quantitative PCR)
Full service gene expression solutions for the post genomic era.
Bionexus specializes in the quantitative
determination of RNA and DNA by real-time TaqMan¨ PCR. We use our proprietary
systems, third party systems, design and validate custom systems on demand.
A high degree of automation allows for rapid
and reliable processing from samples through quantitative analysis.
TAQMAN¨. SERVICE (QUANTITATIVE PCR)
Bionexus TaqMan® Service aims to provide expertise and
custom services in the quantitative analysis of infectious agents, gene expression
and the detection of genetic diseases. The TaqMan® Service provides a platform
for multifaceted, multi-disciplinary research in the market. Our knowledge, expertise
and infrastructure is accessible for interested researchers who seek to implement
state-of-the-art laser guided technology based on the TaqMan® principle
enabling detection and accurate quantification of nucleic acids. Based on
our know-how we are able to rapidly expand the list of available TaqMan®
systems. In addition, our expertise in molecular microbiology and immunology
enables us to meet the needs of a multi-disciplinary research community.
Advantage of the TaqMan®
Expertise and infrastructure on state-of-the-art
laser guided technology based on the TaqMan¨ principle enabling detection and
accurate quantification of Nucleic Acids.
Fully Equipped and Automated Molecular
Biology Laboratories for Sample Handling and TaqMan¨ PCR Reaction Preparation
State-of-Art Laser-guided ABI PRISM
7900 HTA FAST Sequence Detection System
Highly Standardized PCR Process
Over 1200 PCR Reactions within a 24-hour
Integration of 6700 Automated Nucleic
Acid Workstation (Applied Biosystems) into the System
High Capacity and Fully Automated Extractions
of Over 1600 DNA or RNA within a 24-hour periodand is able to
prepare four 96-well TaqMan¨ reaction plates in parallel after a single
TaqMan® PCR Application
Quantitative Analysis of Infectious Agents
Gene Expression and Gene Profiling
Detection of Genetic Diseases.
Provide Platform for Multifaceted, Multi-disciplinary
Research in the Market
Expertise in Molecular Microbiology and
Gene Therapy Distribution and Expression
Drug Response Analysis
Cell Bank Copy Number Analysis
Expression System Development
Residual DNA Analysis
Gene Expression Analysis
Sample Preparation service: These
services cover the whole process of sample preparation, TaqMan¨ analysis
and quantification. If 30 samples are provided, we implement the design
and optimization of new TaqMan¨. PCR systems into the final rates for sample
preparation and TaqMan¨. PCR analysis on a single cDNA sample.(Please
Inquire for Pricing)
Over 400 pre-developed TaqMan® systems
in three applications: Pathogen detection, Gene expression , and Allelic
Over 15,000 pre-developed assays with pre-formed primer
and probe mixes.
Our activity in the field of TaqMan® PCR within the last
5 years has resulted in more than 150 pre-developed TaqMan® systems in
the three applications: pathogen detection, gene expression and allelic discrimination,
mostly in the field of Veterinary Medicine. Most of these systems are available
as pre-developed assays with pre-formed primer and probe mixes.
We have developed over 400 TaqMan¨ PCR systems for animal
species. An additional 15,000 pre-developed TaqMan¨ PCR systems are available from
Ruminants (cattle and sheep)
Sample Requirements Cells (in vitro cultured cells, white blood cells, purified
peripheral blood mononuclear cells (PBMCs): One
to 5 million cells. When FACS-sorted and highly
purified cells are provided, the number may be as low as 100,000 cells. We
have performed TaqMan¨. analysis for gene transcription using blastocysts at
the 32 and 64-cell stage with excellent results.
tissue should be provided in a frozen state on dry ice (stored at-80°C
for up to 1 year). Please provide tissue samples not larger than 100 mg.
Fixed tissues: Two 50 micron sections of paraffin-embedded
tissues are required from solid tissues. A third 50 micron section is required
from non-solid tissues such as lung
tissue. TaqMan¨ analysis were performed on a variety of paraffin-embedded
tissues (i.e. skin, lymph node, GI tract, spleen, liver, lung, brain) which
were up to 20 years of
age without significant loss of PCR signals for the endogenous control (GAPDH
and 18S rRNA) when compared to recently embedded tissue (within 1 year).
Viral RNA Load Determination
DNA Load Quantitation of Viruses, Bacteria, and Parasites
Bionexus provides an integrated service for quantitative analysis
of pathogen loads. We offer a variety of validated RNA based systems for RNA
viruses and DNA based systems for viruses, bacteria, and multicellular parasites.
DNA and RNA extraction is quality controlled with TaqMan® PCR systems to
ensure high extraction efficiency. DNA quantity is measured by targeting single
copy genomic gene sequences. Analytical sensitivity of these systems is in the
range of 5 target molecules and the threshold of sensitivity in a plasma samples
is 50 molecules per ml of plasma. Please contact us for a complete list of pathogen
systems. We can also assist you in custom designing and validating TaqMan®
PCR systems to meet your specific pathogen assay needs.
Performance of the TaqMan® PCR
Between 30 and 60 quantitative TaqMan¨. PCR analysis can be performed
from 1 to 5 million cells and from a tissue sample (20 mg of weight). From a
single 50 micron section of a solid organ (such as brain or lymph node) a minimal
number of 30 quantitative TaqMan¨. PCR analysis are possible.
We offer a considerable amount of expertise on the full process,
from sample preparation to design of optimized systems (Please
Inquire for Pricing).
TaqMan® PCR System Design
Free Service for 50 or more samples:
TaqMan® PCR system design and optimization is provided
without additional charge in projects consisting of 50 or more samples.
TaqMan® Probe and Primers sufficient for 3000 reactions
at a 25µl PCR reaction volume System Optimization:
single PCR product verification
analytical specificity: sequencing of
a TaqMan¨ PCR product
amplification efficiency: six-point standard
curve with a positive control
(5% tolerance to GAPDH reference)
discrimination between complementary and
Standardization of the PCR process
The TaqMan® PCR assay provided by the Applied Biosystems
system provides a unique opportunity to standardize the PCR process. Standardization
is achieved using a TaqMan® system design software(Primer Express, Applied
Biosystems). This software produces TaqMan® systems (matching PCR primers
and TaqMan® probes) which will ultimately require only one PCR cycling
protocol. As a consequence, all systems (i.e. to obtain a cytokine profile
from a single sample) can be run on the same 96 ore 384-well plate. In addition,
all our systems are designed to require the same commercially available PCR
mastermix (Universal Mastermix, Applied Biosystems).