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cDNA Library Construction
	Quantity in Basket: None Code: cDNA Library Construction Price: $0.00
	Regular cDNA Library: Total RNA Isolation mRNA Isolation Library Construction Harvesting primary clones (1000 clones/pool) Arraying 960 spots on Nylon Membranes(Duplicate) Complete Package Subtracted cDNA Library: Total RNA Isolation mRNA Isolation Library Construction Harvesting primary clones (1000 clones/pool) Gridding of 1000 spots on Nylon Membranes Complete Package Normalized/Full Length cDNA Library: Total RNA Isolation mRNA Isolation Library Construction (Inquire) Harvesting primary clones (1000 clones/pool) Gridding of 1000 spots on Nylon Membranes Complete Package(Inquire)
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cDNA Library Construction cDNA Library Construction Bionexus offer the following custom cDNA library construction services from nanogram quantities of mRNA (i.e. laser capture micro dissected samples). In general, PCR based procedures are avoided to prevent PCR related anomalies. Libraries are prepared in the vector of the customer’s choice with over 90-95% recombination efficiency. Regular cDNA Library Starting with small quantities of mRNA (few nanograms!) Uni-directional or Non-directional >107 primary clones Average insert size >2.0 kb Turnaround time 2-4 weeks Subtracted cDNA Library High quality subtracted cDNA libraries for the identification of differentially expressed genes. No PCR based subtraction (PCR amplification and restriction enzyme digestion techniques result into non-specific amplifications and smaller inserts) Q.C. by virtual northern blot analysis, library screening and PCR >2x106 primary clones Average insert size ~1.8kb Turnaround time 4-6 weeks Normalized cDNA Library The normalization process removes the redundant and multiple copied genes, such as ribosomal and other house-keeping genes. This results in the equal representation of low and high copy genes in the library. Both regular and normalized libraries (N0, N1, N2…) are provided Q.C. by virtual northern blot analysis, library screening, PCR and sequencing >106 primary clones < 90-95% recombinants Turnaround time 6-8 weeks Full-length cDNA Library The full length cDNA libraries are constructed by selectively capturing full-length mRNA followed by synthesis of first and second strands. 1x107 primary clones >90-95% recombinants >60-70% full-length genes Q.C. by sequencing Turnaround time 6-8 weeks Clones from all the libraries can be arrayed on nylon membranes. All communications for this Service will be held in the strictest confidence. Contact Bionexus for a price quotation and more information today.