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Klenow Fragment (5 U/ul) Quantity in Basket: None
Code: BN19300
Price: $75.00
Shipping Weight: 0.20 pounds
Untitled Document Size: 200 Units
DESCRIPTION
Klenow Fragment is a proteolytic product of intact DNA polymerase I which retains 5'-3' DNA polymerase and 3'-5' exonuclease activity but lacks 5'-3' exonuclease activity.
SOURCE
Recombinant E. coli strain.
APPLICATIONS
- DNA sequencing by the Sanger dideoxy method.
- Filling-in and/or labeling of recessed 3'-ends.
- Second strand cDNA synthesis.
- End-labeling of DNA molecules with protruding 3' tails.
- Synthesis of dsDNA from ssDNA templates during in vitro mutagenesis.
Unit (U) Definition: One unit is defined as the amount of enzyme required to catalyze the incorporation of 10nmol of dNTPs into acid-insoluble form in 30 minutes at 37°C.
Standard Unit Assay Conditions: 67mM KPO4 (pH7.5), 6.7mM MgCl2, 1mM DTT, 133µM activated calf thymus DNA and 33µM dATP, dCTP, dGTP and [3H]-dTTP.
Concentration: 5-10 units/µl.
10X Reaction Buffer: 100mM Tris-HCl (pH7.5@37°C), 50mM MgCl2, 7.5mM DTT. dNTPs not included
Storage Conditions: 50mM Tris-HCl (pH7.5), 1mM DTT, 0.1mM EDTA and 50% glycerol. Store at -20°C.
ORDERING INFORMATION
BN19300
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