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M-MLV Reverse Transcriptase, 10,000 Units (200 U/ul)
 
  Quantity in Basket: None
Code: BN19500
Price: $63.00
Shipping Weight: 0.20 pounds
 
 
Quantity:
 
Untitled Document Size: 10,000 Units


DESCRIPTION
M-MLV RT (Molony Murine Leukemia Virus Reverse Transcriptase) is an RNA-dependent DNA polymerase requiring a DNA primer and an RNA template to synthesize a complementary DNA strand. M-MLV RT has a weaker intrinsic RNase H activity than AMV RT (Avian Myeloblastosis Virus Reverse Transcriptase). Purified from an E. coli strain carrying a M-MLV RT overproducing plasmid.

APPLICATIONS:
  • First strand cDNA synthesis
  • Primer extension
SOURCE
Recombinant E. coli strain

CONCENTRATION
200 U/ul

STORAGE BUFFER
20 mM Tris-HCl (pH 7.5), 200 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01 % (v/v) Nonidet P-40, 50 % Glycerol
5X reaction buffer

250 mM Tricine-KOH (pH 8.9), 50 mM (NHl4)l2SO4, 100 mM KCl, 50 mM DTT, 15 mM MgCl2

STORAGE CONDITION
-20 °C

FEATURES
M-MLV RT perform superior result, efficiency, sensitivity, and linearity over a wide range of starting RNA amounts.

Quality control tests: Activity, SDS-PAGE/purity, ds-DNase, RNase, endonuclease/nickase, first-strand cDNA synthesis

Activity unit definition: One unit incorporate 1 nmol of deoxynucleotide into acid-precipitable material in 10 min at 37? using poly(A)oligo(dT)12-18 as template-primer.

Purity definition: Single 71 kDa band was visible in SDS-PAGE.




ORDERING INFORMATION

Catalog #
Products
Size
BN19500
M-MLV Reverse Transcriptase (200U/ul)
10,000 U
BN19501
M-MLV Reverse Transcriptase (200U/ul)
20,000 U


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