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Micro Bio Shaker (BNMBR-022UP)
 
Micro Bio Shaker (BNMBR-022UP) Quantity in Basket: None
Code: BNMBR-022UP
Price: $4,250.00
Shipping Weight: 20.00 pounds
 
 
Quantity:
 

Model MBR-022UP Micro Bio Shaker
(Replacement for MBR-022)


Deep Well Maximizer

It holds most existing Universal Well Block in the market


For Square-Well Block (96-well) and kinds of Micro-titer Plates
2 Times More Culture Efficiency
5 Times More Production Efficiency of Plasmid Suit for 2 of Square-Well Block (96-well)


This model can support 2 units of 96 deep well plates with its newly developed holder (PAT.P)

Specifications:

Shaking way: Horizontal Eccentric Vibration
Temp. and Speed display: Digital
Shaking Speed: Strong agitation 300 to 2500 r/min

Program Function: 9 Segments (Temperature and Time)
Plate Capacity: 2 Wellplate (normal, 96deepwell). Also it holds micro plates and micro tubes of (0.5/1.5/2.0 ml), Cat# EM- 1524, EM-0524(Microtube mixed by using a special rack).

Also 2 Units of 96 deep well plates are loadable
Temp. Range Ambient +15oC ~ 65oC
Temp. Accuracy: 0.3 - 1.0oC
Heater: 70 W
Safety Device: High Temp. safety device,Circuit protector, Sensor abnormal signal. High Temp.
Power Supply: AC90-110 V, 2A
Weight: 12 kgDimensions: 270 x 360 x 240Hmm (Its compact size is suitable for a desktop use!!!)
Application Use :

{Prodution of plasmid by culture of E.coli for genomic analysis or forming of DNA array
{Antibrldies response in phage display and ELISA
{Pharmaceuticalmetabolism test
{Dissolution test of refractories
{Preprocessing of nucleic acid refinement

{ For Pre-Treatment of Genome Analysis
{ For Dissolve Test
{ For Pre-Treatment of Nucleotide Extraction
{ Restriction Enzyme digest
{ ELISA
{ Gel Staining + Destaining
{ Immunoassay
{ Microtiter plate hybridization
{ Bacterial transfection
{ Filter washing
Experiment:
The Extraction of plasmid DNA was performed by using MBR-022 with Square well Block (96-well) to culture E.coli. The yield of plasmid DNA was calculated by the OD value of spectrophotometer (OD: 260 nm) and the conclusion of photograph of electrophoresis.
  • The Average values of OD (culture broth 1ml)
    Normal type Incubator 0.54/8 h 0.66/12 h
    MBR-022UP 1.33/8 h 1.45/12 h
  • The Average yields of OD (culture broth 1ml)
    Normal type Incubator 0.66 ug/8 h 0.69 ug/12 h
    MBR-022 2.41 ug/8 h 3.63 ug/12 h


The Figures are shown cultivation time in the scheme.
The growth carve is made by taking average of OD
values in the 5 point
(a ~e).

Conclusion on Electrophoresis of Plasmid DNA

Culture broth (8h)
M is in the right lane as size markers.
a~e are corresponded to each measured
point.

Normal Type Incubator--- MBR--022

Normal Type Incubator-- MBR--022
[E.coli Cultivation]
Container: Square-well Block (96-well)
Sample: JM109 (pUC19): Single colony
Culture: 1ml LB broth (Ampicillin Addictive.
[Extraction of plasmid DNA]
Method: Filtration Kit
[Electrophoresis]
Marker:l DNA-HIND III 0.3 ug
Buffer: TAE, Voltage:120V,30min
Gel: Agarose (1%w/v)
Sample: 3ul of Extracted plasmid DNA (All Amount 50 ul).
Stain: SYBR Green TM(After Electrophoresis).
Additional Product Information