Micro Bio Shaker (BNMBR-022UP)
	Quantity in Basket: None Code: BNMBR-022UP Price: $3,250.00 Shipping Weight: 20.00 pounds
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Model MBR-022UP Micro Bio Shaker (Replacement for MBR-022) Deep Well Maximizer It holds most existing Universal Well Block in the market For Square-Well Block (96-well) and kinds of Micro-titer Plates 2 Times More Culture Efficiency 5 Times More Production Efficiency of Plasmid Suit for 2 of Square-Well Block (96-well) This model can support 2 units of 96 deep well plates with its newly developed holder (PAT.P) Specifications: Shaking way: Horizontal Eccentric Vibration Temp. and Speed display: Digital Shaking Speed: Strong agitation 300 to 2500 r/min Program Function: 9 Segments (Temperature and Time) Plate Capacity: 2 Wellplate (normal, 96deepwell). Also it holds micro plates and micro tubes of (0.5/1.5/2.0 ml), Cat# EM- 1524, EM-0524(Microtube mixed by using a special rack). Also 2 Units of 96 deep well plates are loadable Temp. Range Ambient +15oC ~ 65oC Temp. Accuracy: ± 0.3 - 1.0oC Heater: 70 W Safety Device: High Temp. safety device,Circuit protector, Sensor abnormal signal. High Temp. Power Supply: AC90-110 V, 2A Weight: 12 kgDimensions: 270 x 360 x 240Hmm (Its compact size is suitable for a desktop use!!!) Application Use : {Prodution of plasmid by culture of E.coli for genomic analysis or forming of DNA array {Antibrldies response in phage display and ELISA {Pharmaceuticalmetabolism test {Dissolution test of refractories {Preprocessing of nucleic acid refinement { For Pre-Treatment of Genome Analysis { For Dissolve Test { For Pre-Treatment of Nucleotide Extraction { Restriction Enzyme digest { ELISA { Gel Staining + Destaining { Immunoassay { Microtiter plate hybridization { Bacterial transfection { Filter washing Experiment: The Extraction of plasmid DNA was performed by using MBR-022 with Square well Block (96-well) to culture E.coli. The yield of plasmid DNA was calculated by the OD value of spectrophotometer (OD: 260 nm) and the conclusion of photograph of electrophoresis. The Average values of OD (culture broth 1ml) Normal type Incubator 0.54/8 h 0.66/12 h MBR-022UP 1.33/8 h 1.45/12 h The Average yields of OD (culture broth 1ml) Normal type Incubator 0.66 ug/8 h 0.69 ug/12 h MBR-022 2.41 ug/8 h 3.63 ug/12 h The Figures are shown cultivation time in the scheme. The growth carve is made by taking average of OD values in the 5 point (a ~e). Conclusion on Electrophoresis of Plasmid DNA Culture broth (8h) M is in the right lane as size markers. a~e are corresponded to each measured point. Normal Type Incubator--- MBR--022 Normal Type Incubator-- MBR--022 [E.coli Cultivation] Container: Square-well Block (96-well) Sample: JM109 (pUC19): Single colony Culture: 1ml LB broth (Ampicillin Addictive. [Extraction of plasmid DNA] Method: Filtration Kit [Electrophoresis] Marker:l DNA-HIND III 0.3 ug Buffer: TAE, Voltage:120V,30min Gel: Agarose (1%w/v) Sample: 3ul of Extracted plasmid DNA (All Amount 50 ul). Stain: SYBR Green TM(After Electrophoresis). Additional Product Information