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Model MBR-024 Micro Bio Shaker
(Replacement for M-36)
Option suiting for
Kinds of Well Block
of Each Company |
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For Square-Well Block (96-well) and kinds of Micro-titer Plates
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2 Times More Culture Efficiency
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| 5 Times More Production Efficiency of Plasmid
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Suit for 4 of Square-Well Block (96-well)
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Specifications:
Shaking way: Orbital
Temp. and Speed display: Digital
Shaking Speed: 100 to 1000 r/min
Plate Capacity: 4 Wellplate (normal, deepwell)
Temp. Range Ambient +5oC ~ 65oC
Temp. Accuracy: ± 0.3 - 1.0oC
Timer: 60 minutes
Heater: 80 W
Safety Device: High Temp. safety device,Circuit
protector, Sensor abnormal signal. High Temp.
Power Supply: AC90-110 V, 2A
Weight: 15 kg
Application Use :
{ For Pre-Treatment of Genome Analysis
{ For Dissolve Test
{ For Pre-Treatment of Nucleotide Extraction
{ Restriction Enzyme digest
{ ELISA
{ Gel Staining + Destaining
{ Immunoassay
{ Microtiter plate hybridization
{ Bacterial transfection
{ Filter washing |
Experiment:
The Extraction of plasmid DNA was performed
by using MBR-024 with Square well Block
(96-well)
to culture E.coli. The yield of plasmid
DNA
was calculated by the OD value of spectrophotometer
(OD: 260 nm) and the conclusion of
photograph
of electrophoresis.
- The Average values of OD (culture broth 1ml)
Normal type Incubator 0.54/8 h 0.66/12
h
MBR-024 1.33/8 h 1.45/12 h
- The Average yields of OD (culture broth 1ml)
Normal type Incubator 0.66 ug/8 h 0.69
ug/12
h
MBR-024 2.41 ug/8 h 3.63 ug/12 h
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The Figures are shown cultivation time in
the scheme.
The growth carve is made by taking
average
of OD
values in the 5 point
(a ~e).
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Conclusion on Electrophoresis of Plasmid
DNA
Culture broth (8h)
M is in the right lane as size markers.
a~e are corresponded to each measured
point.
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Normal Type Incubator--- MBR--024
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Normal Type Incubator-- MBR--024 |
[E.coli Cultivation]
Container: Square-well Block (96-well)
Sample: JM109 (pUC19): Single colony
Culture: 1ml LB broth (Ampicillin Addictive.
[Extraction of plasmid DNA]
Method: Filtration Kit
[Electrophoresis]
Marker:l DNA-HIND III 0.3 ug
Buffer: TAE, Voltage:120V,30min
Gel: Agarose (1%w/v)
Sample: 3ul of Extracted plasmid DNA
(All
Amount 50 ul).
Stain: SYBR GreenTM (After Electrophoresis).
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