CUSTOM
MUTAGENESIS SERVICES |
|
Mutagenesis
methodologies labor intensive and technically difficult to perform. In
vitro site-directed mutagenesis is an important methodology used to
study protein structure-function relationships, identify intramolecular regions
or amino acids that may mediate these functions, analyze gene expression and
modify vectors. Bionexus experienced team is able to finish any mutagenesis
project at very fast turnaround time and at a very affordable price.
Method:
Using PCR site-directed method in conjunction with our proprietary protocols.
The mutated region of the sequence is double strand sequenced. Sequence the mutated region by automated
fluorescent sequencing.
Starting Material:
A 20 ug of plasmid DNA submitted by the customer and a hard copy of the restriction
map and the sequence with the indication of the bases that need to be changed.
Result:
A 20 ug of purified plasmid DNA with the correct changes and a complete report
of strategies and data.
Turnaround time:
Each mutant will take 1-2 weeks, including both strands sequence verification.
Please contact us for a discounted price, if you have more than one mutants
to be made.
Ordering
:
- Make sure that the correct changes
are indicated on the sequence printout of the construct. Email modified
sequence and original sequence.
Enclose 20 ug plasmid DNA, vector map and a diskette in a shipping
envelope.
All communications for the Gene synthesis Services will be held in the
strictest confidence.
Contact Bionexus for a
price quotation today.
