cDNA Library

cDNA Library Construction

Bionexus provide a range of cDNA libraries construction services, including standard cDNA library, subtractive cDNA library, normalized cDNA library, full length cDNA library and more.

cDNA Library Construction
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cDNA Library Construction cDNA Library Construction Bionexus constructs full-length cDNA libraries from selectively captured full-length mRNAs using a proprietary technology(i.e. laser capture micro dissected samples). Using a novel reverse transcriptase which successfully transcribes RNA that is even larger than 15 kb the first strands are synthesized with oligo dT primer. The first and second strands are synthesized using special chemistry that abolishes secondary structures and interruptions usually encountered due to high GC rich regions present in the template. In general, PCR based procedures are avoided to prevent PCR related anomalies. Libraries are prepared in the vector of the customer’s choice with over 90-95% recombination efficiency. Regular cDNA Library Starting with small quantities of mRNA (few nanograms!) Uni-directional or Non-directional >107 primary clones Average insert size >2.0 kb Turnaround time 2-4 weeks The following options are available:  Total RNA Isolation mRNA Isolation Library Construction Harvesting primary clones (1000 clones/pool) Arraying 960 spots on Nylon Membranes(Duplicate) Complete Package Subtracted cDNA Library High quality subtracted cDNA libraries for the identification of differentially expressed genes. No PCR based subtraction (PCR amplification and restriction enzyme digestion techniques result into non-specific amplifications and smaller inserts) Q.C. by virtual northern blot analysis, library screening and PCR >2x106 primary clones Average insert size ~1.8kb Turnaround time 4-6 weeks The following options are available:  Total RNA Isolation mRNA Isolation Library Construction Harvesting primary clones (1000 clones/pool) Gridding of 1000 spots on Nylon Membranes Complete Package Normalized cDNA Library The normalization process removes the redundant and multiple copied genes, such as ribosomal and other house-keeping genes. This results in the equal representation of low and high copy genes in the library. Both regular and normalized libraries (N0, N1, N2…) are provided Q.C. by virtual northern blot analysis, library screening, PCR and sequencing >106 primary clones < 90-95% recombinants Turnaround time 6-8 weeks The following options are available:  Total RNA Isolation mRNA Isolation Library Construction Harvesting primary clones (1000 clones/pool) Gridding of 1000 spots on Nylon Membranes Complete Package Full-length cDNA Library The full length cDNA libraries are constructed by selectively capturing full-length mRNA followed by synthesis of first and second strands. 1x107 primary clones >90-95% recombinants >60-70% full-length genes Q.C. by sequencing Turnaround time 6-8 weeks Clones from all the libraries can be arrayed on nylon membranes. All communications for this Service will be held in the strictest confidence. Contact Bionexus for a price quotation and more information today.