- Total RNA Isolation
- mRNA Isolation
- Library Construction
- Harvesting primary clones (1000 clones/pool)
- Arraying 960 spots on Nylon Membranes(Duplicate)
- Complete Package
Subtracted cDNA Library
High quality subtracted cDNA libraries for the identification of differentially
expressed genes.
- No PCR based subtraction (PCR amplification and restriction enzyme digestion
techniques result
into non-specific amplifications and smaller inserts)
- Q.C. by virtual northern blot analysis, library screening and PCR
- >2x106 primary clones
- Average insert size ~1.8kb
- Turnaround time 4-6 weeks
The following options are available:
- Total RNA Isolation
- mRNA Isolation
- Library Construction
- Harvesting primary clones (1000 clones/pool)
- Gridding of 1000 spots on Nylon Membranes
- Complete Package
Normalized cDNA Library
The normalization process removes the redundant and multiple copied genes, such
as ribosomal and other house-keeping genes. This results in the equal representation
of low and high copy genes in the library.
- Both regular and normalized libraries (N0, N1, N2…) are provided
- Q.C. by virtual northern blot analysis, library screening, PCR and sequencing
- >106 primary clones
- < 90-95% recombinants
- Turnaround time 6-8 weeks
The following options are available:
- Total RNA Isolation
- mRNA Isolation
- Library Construction
- Harvesting primary clones (1000 clones/pool)
- Gridding of 1000 spots on Nylon Membranes
- Complete Package
Full-length cDNA Library
The full length cDNA libraries are constructed by selectively capturing full-length
mRNA followed by synthesis of first and second strands.
- 1x107 primary clones
- >90-95% recombinants
- >60-70% full-length genes
- Q.C. by sequencing
- Turnaround time 6-8 weeks
Clones from all the libraries can be arrayed on nylon
membranes.
All communications for this Service will be held in the strictest confidence.
Contact Bionexus for a price
quotation and more information today.